276 bp amplicon (Bio-Rad)
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276 bp amplicon
276 Bp Amplicon, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/276+bp+amplicon/Lyphochek+Whole+Blood+Immunosuppressant+Control/pmc03795701-62-70-8
Average 93 stars, based on 21 article reviews
276 Bp Amplicon, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/276+bp+amplicon/Lyphochek+Whole+Blood+Immunosuppressant+Control/pmc03795701-62-70-8
Average 93 stars, based on 21 article reviews
276 bp amplicon - by Bioz Stars,
2026-09
93/100 stars
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Polymerase Chain Reaction:Article Title: Caspase-mediated apoptosis in crustaceans: cloning and functional characterization of EsCaspase-3-like protein from Eriocheir. Article Snippet: The caspase-3-like gene was cloned from Eriocheir sinensis, and its properties were characterized to identify the biological implications of this caspase in apoptosis in crab.. Its deduced full-length protein sequence consists of 462 amino acid residues, including the prodomain and the large and small subunits.. Moreover, several residues known to be critical in the caspase-3 catalytic center and binding pocket, as well as the active site pentapeptide motif Q220ACRG224, were identically present in the deduced EsCaspase-3-like protein. Article Title: Association of a Hepatopancreas-Specific C-Type Lectin with the Antibacterial Response of Eriocheir sinensis Article Snippet: Quantitative RT-PCR was performed using SYBR ® Premix ExTaq TM (TaKaRa) with a EsLecF gene-specific primer pair ( ) producing a 231-bp amplicon. .. PCR was carried out in a CFX96 instrument (Bio-Rad, Hercules, CA, USA) as follows: 30 cycles at 94°C for 30 s, 58°C for 30 s, and 72°C for 1 min. Internal control PCR reactions for β-actin were performed in a separate tube, as described above with the exception of an alternative gene-specific primer pair , which was designed based upon a cloned E. sinensis β-actin cDNA fragment to produce a Control:Article Title: Caspase-mediated apoptosis in crustaceans: cloning and functional characterization of EsCaspase-3-like protein from Eriocheir. Article Snippet: The caspase-3-like gene was cloned from Eriocheir sinensis, and its properties were characterized to identify the biological implications of this caspase in apoptosis in crab.. Its deduced full-length protein sequence consists of 462 amino acid residues, including the prodomain and the large and small subunits.. Moreover, several residues known to be critical in the caspase-3 catalytic center and binding pocket, as well as the active site pentapeptide motif Q220ACRG224, were identically present in the deduced EsCaspase-3-like protein. Article Title: Association of a Hepatopancreas-Specific C-Type Lectin with the Antibacterial Response of Eriocheir sinensis Article Snippet: Quantitative RT-PCR was performed using SYBR ® Premix ExTaq TM (TaKaRa) with a EsLecF gene-specific primer pair ( ) producing a 231-bp amplicon. .. PCR was carried out in a CFX96 instrument (Bio-Rad, Hercules, CA, USA) as follows: 30 cycles at 94°C for 30 s, 58°C for 30 s, and 72°C for 1 min. Internal control PCR reactions for β-actin were performed in a separate tube, as described above with the exception of an alternative gene-specific primer pair , which was designed based upon a cloned E. sinensis β-actin cDNA fragment to produce a Clone Assay:Article Title: Caspase-mediated apoptosis in crustaceans: cloning and functional characterization of EsCaspase-3-like protein from Eriocheir. Article Snippet: The caspase-3-like gene was cloned from Eriocheir sinensis, and its properties were characterized to identify the biological implications of this caspase in apoptosis in crab.. Its deduced full-length protein sequence consists of 462 amino acid residues, including the prodomain and the large and small subunits.. Moreover, several residues known to be critical in the caspase-3 catalytic center and binding pocket, as well as the active site pentapeptide motif Q220ACRG224, were identically present in the deduced EsCaspase-3-like protein. Article Title: Association of a Hepatopancreas-Specific C-Type Lectin with the Antibacterial Response of Eriocheir sinensis Article Snippet: Quantitative RT-PCR was performed using SYBR ® Premix ExTaq TM (TaKaRa) with a EsLecF gene-specific primer pair ( ) producing a 231-bp amplicon. .. PCR was carried out in a CFX96 instrument (Bio-Rad, Hercules, CA, USA) as follows: 30 cycles at 94°C for 30 s, 58°C for 30 s, and 72°C for 1 min. Internal control PCR reactions for β-actin were performed in a separate tube, as described above with the exception of an alternative gene-specific primer pair , which was designed based upon a cloned E. sinensis β-actin cDNA fragment to produce a Amplification:Article Title: Caspase-mediated apoptosis in crustaceans: cloning and functional characterization of EsCaspase-3-like protein from Eriocheir. Article Snippet: The caspase-3-like gene was cloned from Eriocheir sinensis, and its properties were characterized to identify the biological implications of this caspase in apoptosis in crab.. Its deduced full-length protein sequence consists of 462 amino acid residues, including the prodomain and the large and small subunits.. Moreover, several residues known to be critical in the caspase-3 catalytic center and binding pocket, as well as the active site pentapeptide motif Q220ACRG224, were identically present in the deduced EsCaspase-3-like protein. Article Title: Association of a Hepatopancreas-Specific C-Type Lectin with the Antibacterial Response of Eriocheir sinensis Article Snippet: Quantitative RT-PCR was performed using SYBR ® Premix ExTaq TM (TaKaRa) with a EsLecF gene-specific primer pair ( ) producing a 231-bp amplicon. .. PCR was carried out in a CFX96 instrument (Bio-Rad, Hercules, CA, USA) as follows: 30 cycles at 94°C for 30 s, 58°C for 30 s, and 72°C for 1 min. Internal control PCR reactions for β-actin were performed in a separate tube, as described above with the exception of an alternative gene-specific primer pair , which was designed based upon a cloned E. sinensis β-actin cDNA fragment to produce a |